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DNA 甲基化分析試劑
目前已知異常的 DNA 甲基化程度與多種癌症、疾病具有高度相關性,因此可藉由找尋調控此機制的方式,開發具有潛力的治療藥物。以下我們將為您介紹針對偵測 Genomic DNA 甲基化程度所設計的便利試劑組 "DNA Methylation Detection Kit",以及各種相關抗體、抑制劑與活性分析試劑產品。同時我們也將介紹如何利用 Revvity 公司特殊研發的 Alpha 技術,建立起全均質化的快速分析平台。歡迎多加利用便利的伯森客戶服務資源 02-2788-2121 / 0800-059-668 / 線上留言,讓我們協助您快速取得適用產品資訊。
- DNA Methylation Detection Kit
- Antibodies, Inhibitors, Activity Assay Kits
- Alpha Assays
DNA Methylation Detection Kit
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針對偵測 Genomic DNA 甲基化程度所設計的便利試劑組,可於三小時內完成亞硫酸鹽處理步驟。
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亞硫酸鹽 (bisulfite, NaHSO3) 具有能夠將 CpG 裡的 Cytosine 轉變為 Uracil (使得原本兩股互補的 DNA 序列形成無法互補配對的單股序列),以及將 mCpG 裡的 5-Methyl Cytosine 轉變為 Cytosine 的特性 (可維持原本兩股互補配對狀態)。經亞硫酸鹽處理過的 Genomic DNA 樣本可透過甲基化專一性 PCR (Methylation Specific PCR, MSP)、定序或微陣列晶片等…方式,測得 Genomic DNA 的甲基化程度。
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每套產品可供進行 50 個反應,每個反應可處理 500 pg ~ 2 µg Genomic DNA。
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產品特性包含:
- Provides a true endogenous human positive control which eliminates any ambiguity in validating results. The positive control has been tested in over 15 different human tissues.
- Our protocol guarantees > 99 % conversion of cytosine to uracil and > 99 % CpG protection, as calculated by sequencing.
- Users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarrays.
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訂購資訊
Product
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Pack Size |
Cat. No. |
DNA Methylation Detection Kit |
1 kit
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BC-K5082100
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Antibodies, Inhibitors, Activity Assay Kits
Enzo Life Sciences 公司針對 Epigenetics 研究推出了一系列完整的分析工具,以下即為您摘錄 DNA Methylation 相關研究試劑。由於品項眾多無法一一詳列,歡迎洽詢伯森業務專員索取完整產品資訊。 |
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Antibodies (點擊「產品名稱」,即可開啟詳細產品說明)
Product |
Pack Size |
Cat. No. |
Reactivity |
Application |
Dnmt1, mAb (60B1220.1) |
100 µg |
ALX-804-369-C100 |
H, M, Z |
ICC, IHC, IP, ChIP, WB |
Dnmt3a (mouse), mAb (64B1446) |
100 µg |
ALX-804-370-C100 |
M |
ICC, IHC, IP, ChIP, WB |
Dnmt3b, mAb (52A1018) |
100 µg |
ALX-804-233-C100 |
H, M |
ICC, IHC, IP, ChIP, WB |
ChIP: Chromatin Immunoprecipitation
, H: Human, ICC: Immunocytochemistry, IHC: Immunohistochemistry, IP: Immunoprecipitation, M: Mouse, WB: Western Blot, Z: Zebrafish |
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Inhibitors (點擊「產品名稱」,即可開啟詳細產品說明)
Product |
Description |
Pack Size |
Cat. No. |
5-Aza-2'-deoxycytidine |
DNA methyltransferase inhibitor |
5 mg |
ALX-480-096-M005 |
(-)-Epigallocatechin gallate |
Inhibitor of NOS, telomerase and Dnmt |
10 mg |
ALX-270-263-M010 |
50 mg |
ALX-270-263-M050 |
Zebularine |
Dnmt inhibitor |
10 mg |
BML-GR344-0010 |
Epigenetics library |
The Screenwell™ Epigenetics library contains 43 inhibitors of these important enzymes: HDACs, SIRTs, Lysine demethylases, HATs, Histone methyl transferases, DNA methyltransferases as well as SIRT activators.
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1 Library, 100 µl/well |
BML-2836-0100 |
1 Library, 500 µl/well |
BML-2836-0500 |
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Activity Assay Kits (點擊「產品名稱」,即可開啟詳細產品說明)
Product |
Description |
Pack Size |
Cat. No. |
Methyltransferase activity kit |
Fluorescent detection for any S-adenosyl-L-methionine (AdoMet/SAM) dependent methyltransferase |
96 wells |
ADI-907-025 |
Methyltransferase HT activity kit |
Fluorescent detection for any S-adenosyl-L-methionine (AdoMet/SAM) dependent methyltransferase
This kit is ideal for high throughout (HT) applications as the SAM is provided separately giving you the flexibility to titrate the SAM to be in excess for your specific MT of interest |
96 wells |
ADI-907-032 |
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Alpha for DNA Methylation Assays
「Alpha Toolbox」以及「AlphaScreen Fusion Tag Detection Kit」系列,是由 Alpha 技術所延伸出來的便利產品,能夠自由組合出多種偵測平台,以取代步驟繁複的 PCR、EMSA、ChIP、ELISA 等類型產品,讓研究者僅需使用單一技術就能進行 DNA methylation、DNA:Protein interaction、Histone modification 等多項表觀遺傳學分析實驗。以下我們就以 DNA Methyltransferase 1 (DNMT1) 酵素活性分析實驗為例,介紹如何利用 Alpha 技術建立起簡單快速的分析平台。
偵測原理與步驟:
Alpha 技術主要包含有兩個核心試劑,分別是 Donor bead 與 Acceptor bead。在進行反應時,僅需藉由特定的分子結合將兩者拉近,即可在 680 nm 雷射光激發下發出 615 nm (AlphaLISA acceptor bead) 或 520-620 nm (AlphaScreen acceptor bead) 的螢光訊號。
在 DNMT1 酵素活性分析實驗中,僅需準備下列試劑即可進行實驗。流程步驟則如右圖所示。
- Alpha Donor Bead, streptavidin-coated
- AlphaLISA Acceptor Bead, conjugated with anti-tag antibody
- DNMT1 enzyme
- S-adenosylmethionine (SAM)
- Biotinylated oligonucleotide, contained 10 CpG methylation sites on each strand
- Tagged-Methyl Binding Protein (MeBP)
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Alpha 技術不僅流程簡單快速,還能提供精確量化的實驗分析結果。分別在 30 µM SAM 與 600 µM SAM 濃度條件下,使用已知的 DNMT1 活性促進劑 "DMSO",以及已知的 DNMT1 抑制劑 "Sinefungin" 與 "SAH",測試 Alpha 平台的偵測能力。結果證實 Alpha 技術的確能夠專一且準確地進行酵素活性偵測。
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Alpha Toolbox & AlphaScreen Fusion Detection Kit
Alpha Toolbox 與 AlphaScreen Fusion Detection Kit 系列產品,可以讓研究者自由組合出多種偵測平台,以彈性符合多元化的實驗需求與生物樣本特性。(點擊下表品項,即可開啟詳細產品說明)
Alpha Toolbox |
AlphaScreen Fusion Tag
Detection Kit1 |
Alpha Donor Bead
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AlphaLISA Accetor Bead
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(註1)
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Kit 中皆含有 AlphaScreen Streptavidin coated Donor Bead 與 AlphaScreen anti-tag antibody coated Acceptor Bead。 |
(註2) |
可作為 Alpha 實驗中的 Positive control 使用。 |
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AlphaScreen Acceptor Bead
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訂購資訊 (請點擊下列分類選項,瀏覽詳細訂購資訊)
Alpha Donor Bead |
Product |
Pack Size |
Cat. No. |
Anti-FLAG Alpha Donor Beads |
1 mg |
PK-AS103D |
5 mg |
PK-AS103M
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25 mg |
PK-AS103R |
Anti-Mouse IgG Alpha Donor Beads |
1 mg |
PK-AS104D |
5 mg |
PK-AS104M
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25 mg |
PK-AS104R |
Anti-Rabbit IgG Alpha Donor Beads |
1 mg |
PK-AS105D |
5 mg |
PK-AS105M
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25 mg |
PK-AS105R |
AlphaScreen Glutathione Donor Beads |
1 mg |
PK-6765300 |
5 mg |
PK-6765301
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25 mg |
PK-6765302 |
AlphaScreen Nickel Chelate Donor Beads |
1 mg |
PK-AS101D |
5 mg |
PK-AS101M
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25 mg |
PK-AS101R |
Protein A Alpha Donor Beads |
1 mg |
PK-AS102D |
5 mg |
PK-AS102M
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25 mg |
PK-AS102R |
Strep-Tactin Alpha Donor Beads |
1 mg |
PK-AS106D |
5 mg |
PK-AS106M
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25 mg |
PK-AS106R |
AlphaScreen Streptavidin Donor Beads |
1 mg |
PK-6760002S |
5 mg |
PK-6760002 |
50 mg |
PK-6760002B |
AlphaScreen Unconjugated Donor Beads |
1 mg |
PK-6762013 |
5 mg |
PK-6762011 |
50 mg |
PK-6762012 |
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AlphaLISA Acceptor Bead |
Product |
Pack Size |
Cat. No. |
Anti-6xHis AlphaLISA Acceptor Beads |
250 µg |
PK-AL128C |
5 mg |
PK-AL128M |
50 mg |
PK-AL128R |
Anti-Chicken IgY AlphaLISA Acceptor Beads |
250 µg |
PK-AL131C |
5 mg |
PK-AL131M |
50 mg |
PK-AL131R |
Anti-c-Myc AlphaLISA Acceptor Beads |
250 µg |
PK-AL111C |
5 mg |
PK-AL111M |
50 mg |
PK-AL111R |
Anti-DIG AlphaLISA Acceptor Beads |
250 µg |
PK-AL113C |
5 mg |
PK-AL113M |
50 mg |
PK-AL113R |
Anti-FITC AlphaLISA Acceptor Beads |
250 µg |
PK-AL127C |
5 mg |
PK-AL127M |
50 mg |
PK-AL127R |
Anti-FLAG AlphaLISA Acceptor Beads |
250 µg |
PK-AL112C |
5 mg |
PK-AL112M |
50 mg |
PK-AL112R |
Anti-GFP AlphaLISA Acceptor Beads |
250 µg |
PK-AL133C |
5 mg |
PK-AL133M |
50 mg |
PK-AL133R |
Anti-Goat IgG (Fc specific) AlphaLISA Acceptor Beads |
250 µg |
PK-AL107C |
5 mg |
PK-AL107M |
50 mg |
PK-AL107R |
Anti-GST AlphaLISA Acceptor Beads |
250 µg |
PK-AL110C |
5 mg |
PK-AL110M |
50 mg |
PK-AL110R |
Anti-Human IgG (Fc-specific) AlphaLISA Acceptor Beads |
250 µg |
PK-AL103C |
5 mg |
PK-AL103M |
50 mg |
PK-AL103R |
Anti-Maltose-Binding Protein (MBP) AlphaLISA Acceptor Beads |
250 µg |
PK-AL134C |
5 mg |
PK-AL134M |
50 mg |
PK-AL134R |
Anti-Mouse IgG (Fc-specific) AlphaLISA Acceptor Beads |
250 µg |
PK-AL105C |
5 mg |
PK-AL105M |
50 mg |
PK-AL105R |
Anti-Mouse IgM AlphaLISA Acceptor Beads |
250 µg |
PK-AL130C |
5 mg |
PK-AL130M |
50 mg |
PK-AL111R |
Anti-Rabbit IgG (Fc-specific) AlphaLISA Acceptor Beads |
250 µg |
PK-AL104C |
5 mg |
PK-AL104M |
50 mg |
PK-AL104R |
Anti-Rat IgG (Fc-specific) AlphaLISA Acceptor Beads |
250 µg |
PK-AL106C |
5 mg |
PK-AL106M |
50 mg |
PK-AL106R |
Anti-Sheep IgG (Fc specific) AlphaLISA Acceptor Beads |
250 µg |
PK-AL132C |
5 mg |
PK-AL132M |
50 mg |
PK-AL132R |
Anti-V5 AlphaLISA Acceptor Beads |
250 µg |
PK-AL129C |
5 mg |
PK-AL129M |
50 mg |
PK-AL129R |
Glutathione AlphaLISA Acceptor Beads |
250 µg |
PK-AL109C |
5 mg |
PK-AL109M |
50 mg |
PK-AL109R |
Nickel Chelate AlphaLISA Acceptor Beads |
250 µg |
PK-AL108C |
5 mg |
PK-AL108M |
50 mg |
PK-AL108R |
Protein A AlphaLISA Acceptor Beads |
250 µg |
PK-AL101C |
5 mg |
PK-AL101M |
50 mg |
PK-AL101R |
Protein G AlphaLISA Acceptor Beads |
250 µg |
PK-AL102C |
5 mg |
PK-AL102M |
50 mg |
PK-AL102R |
Protein L AlphaLISA Acceptor Beads |
250 µg |
PK-AL126C |
5 mg |
PK-AL126M |
50 mg |
PK-AL126R |
Strep-Tactin AlphaLISA Acceptor Beads |
250 µg |
PK-AL136C |
5 mg |
PK-AL136M |
50 mg |
PK-AL136R |
Streptavidin AlphaLISA Acceptor Beads |
250 µg |
PK-AL125C |
5 mg |
PK-AL125M |
50 mg |
PK-AL125R |
Unconjugated AlphaLISA Acceptor Beads |
1 mg |
PK-6772001 |
5 mg |
PK-6772002 |
50 mg |
PK-6772003 |
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AlphaScreen Acceptor Bead |
Product |
Pack Size |
Cat. No. |
AlphaScreen Protein A Acceptor Beads |
5 mg |
PK-6760137M |
50 mg |
PK-6760137R
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AlphaScreen Unconjugated Acceptor Beads |
1 mg |
PK-6762003 |
5 mg |
PK-6762001 |
50 mg |
PK-6762002 |
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AlphaScreen Fusion Tag Detection Kit |
Product |
Pack Size |
Cat. No. |
AlphaScreen c-Myc Detection Kit |
500 assay |
PK-6760611C |
10,00 assay |
PK-6760611M
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50,00 assay |
PK-6760611R |
AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit |
500 assay |
PK-6760604C |
10,00 assay |
PK-6760604M
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50,00 assay |
PK-6760604R |
AlphaScreen FITC (Fluorescein) Detection Kit |
500 assay |
PK-6760605C |
10,00 assay |
PK-6760605M
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50,00 assay |
PK-6760605R |
AlphaScreen FLAG Detection Kit |
500 assay |
PK-6760613C |
10,00 assay |
PK-6760613M
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50,00 assay |
PK-6760613R |
AlphaScreen GST Detection Kit |
500 assay |
PK-6760603C |
10,00 assay |
PK-6760603M
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50,00 assay |
PK-6760603R |
AlphaScreen HA (Hemagglutinin) Detection Kit |
500 assay |
PK-6760612C |
10,00 assay |
PK-6760612M
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50,00 assay |
PK-6760612R |
AlphaScreen Histidine (Nickel Chelate) Detection Kit |
500 assay |
PK-6760619C |
10,00 assay |
PK-6760619M
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50,00 assay |
PK-6760619R |
AlphaScreen Biotinylated-GST |
10,00 assay |
PK-6760305M |
AlphaScreen Biotinylated-HIS |
10,00 assay |
PK-6760303M |
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Other Alpha Products |
Classification |
Product |
Pack Size |
Cat. No. |
AlphaLISA Buffers |
AlphaLISA Dissociation Buffer, 3X |
10 ml |
PK-AL006C |
100 ml |
PK-AL006F |
AlphaLISA HiBlock Buffer, 10X |
10 ml |
PK-AL004C |
100 ml |
PK-AL004F |
AlphaLISA Immunoassay Buffer, 10X |
10 ml |
PK-AL000C |
100 ml |
PK-AL000F |
AlphaLISA Lysis Buffer, 5X |
10 ml |
PK-AL003C |
100 ml |
PK-AL003F |
AlphaLISA NaCl Buffer, 5X |
10 ml |
PK-AL007C |
100 ml |
PK-AL007F |
AlphaLISA Universal Buffer, 5X |
10 ml |
PK-AL001C |
100 ml |
PK-AL001F |
Bead Conjugation Kit |
AlphaScreen Conjugation Kit
Each Kit Contains: Streptavidin Alpha Donor Bead, unconjugated AlphaScreen Acceptor Bead. |
2 x 2 mg |
PK-6760000K |
Optimization Tools
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AlphaScreen Omnibeads
OmniBead are designed as a tool to troubleshoot signal variability. The Bead contain all of the chemical components needed to generate a strong AlphaScreen signal without the presence of AlphaScreen Donor or Acceptor Bead. |
1,000 assay |
PK-6760626D |
10,00 assay |
PK-6760626M
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50,00 assay |
PK-6760626R |
AlphaScreen TruHits Kit
Each Kit Contains: Biotinylated Acceptor Bead, streptavidin Alpha Donor Bead for identifying false positives. |
1,000 assay |
PK-6760627D |
10,00 assay |
PK-6760627M
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