蛋白質表現與分析

　二維電泳膠樣本處理與膠體染色 (2-D Electrophoresis Sample Preparation and Gel Staining)
　SYPRO Ruby Gel Stain--螢光膠片染色法


　High Sensitivity：
　靈敏度為1~2 ng，相當於最佳化的銀染靈敏度。

　Easy to Use：
　溶液隨開隨用不需再配製。染色過程不需
　終止反應與退染。

　Versatile：
　可適用於質譜分析與序列分析樣本之染色。
　也適用於醣基化蛋白(Glycosylated Protein)、
　脂蛋白(Lipoprotein)、鈣結合蛋白(Calcium
　Binding Protein)及其他難以染色的蛋白質。

　Rapid：
　可在最短時間內(最快4 hr)得到最好的效果。

　Quantitative：
　線性範圍大a 0~1000 ng (傳統銀染僅為0~80 ng)。

　Ex/Em：
　280, 450/610 nm

　

　SYPRO Ruby protein gel stain shows less protein- to-protein
　variation than silver staining. Proteins from a cell lysate were
　run on a 2-D gel and stained with SYPRO Ruby protein gel
　stain (left) or silver stain (right). The grayscale values of the
　SYPRO Ruby dye- stained gel have been inverted for easier
　comparison with the silver-stained gel.


Amounts of carbonic anhydrase ranging
from 1 ng to 1000 ng were separated on
an SDS-polyacrylamide gel and stained
with the SYPRO Ruby protein gel stain
The inset shows the excellent linearity in
the lower part of the range from 1 ng to
60 ng protein. Staining intensities were
quantitated using the Bio-Rad Molecular
Imager FX System. For comparison, the
gray band shows the linear range for the
same protein detected with silver staining.


訂購資訊：
目錄號碼
產品
包裝
S-12000
Sypro Ruby Gel Stain
1 L/bottle
S-21900
Sypro Ruby Gel Stain
5 L/bottle