核酸純化、合成與分析
　核酸萃取 (Nucleic Acid Extraction)

　Plasmid Miniprep96 Kit


　產品特點：
　•不需離心或沈澱，實驗流程僅需25分鐘
　•萃取出的Plasmid DNA可直接應用於Cloning、
　　Sequencing、Transformation、PCR等實驗
　•96-well format設計，可輕鬆快速的處理大量samples


　簡易的操作流程：

　可信賴的再現性結果：
Host strain Plasmid Yield (μg)
XL 1-blue pUC19 2.1 - 6.1
  pLH2** 3.5 - 9.2
JM109 pUC19 2.4 - 6.4
  pLH2** 3.5 - 9.1
Results will vary depending on host strain, plasmid, and protocol conditions.** pLH2 was derived from pUC19. The 2.0 Kb fragment of Hind III digested l phage was cloned into the Hind III site of pUC19, resulting in a 4.7 Kb plasmid.
表一､Montage Plasmid Miniprep96 Kit平均的DNA yields介於 4∼6 ug之間（From 1 mL bacterial culture ）



圖一､Montage Plasmid Miniprep96 Kit抽取出的 plasmid DNA電泳圖。 可觀察到不同well之間所抽取出的plasmid DNA 量並沒有太大差異。

　Kit Components

 

Part Function
　A. 96-well deep well culture block 　Growth of host bacteria
　B. PLASMID plate 　Purification of plasmid DNA
　C. Lysate clearing plate 　Clearing of bacterial lysate
　D. V-bottom storage plate 　Storage of plasmid DNA samples
　E. Sealing tape 　Sealing of V-bottom storage plate
　F. RNase A 　Required additive for Solution 1
　G. Solution 1 　Cell resuspension
　H. Solution 2 　Cell lysis
　I. Solution 3 　Neutralization
　J. Solution 4 　Wash　
　K. Solution 5 　Resuspension / storage of plasmid DNA



　訂購資訊：

目錄號碼 產品 包裝
LSKP 096 01 Montage Plasmid Miniprep96 Kit 1 x 96 plasmid miniprep
LSKP 096 04 Montage Plasmid Miniprep96 Kit 4 x 96 plasmid minipreps
LSKP 096 24 Montage Plasmid Miniprep96 Kit 24 x 96 plasmid minipreps


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