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基因表現與分析
報導基因表現分析 (Reporter
Gene Analysis)
β-Galactosidase Assay System
本公司共有三種冷光試劑套組可用來偵測β-Galactosidase的活性，簡單的比較表如下：
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Galacto-Light
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Galacto-Light Plus
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Galacto-Star
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需要分注器的冷光儀
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Yes
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No
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No
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流程
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依序加入Reaction buffer and Enhancer
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One Step
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樣品種類
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Mammalian Cell
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Yeast or Mammalian Cell
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內生性β-Galactosidase的干擾
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No
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No
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Possible
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Cost
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Low
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Medium
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High
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冷光試劑套組具備下列優點：
•High Assay Range：毫微微克(fg)到毫微克(ng)範圍內正確測量酵素量
•High Sensitivity：敏感度是放射性或非放射性chloramphenicol acetyl transferase
(CAT) 或顯色法及螢光
法測量的百倍到千倍
•Wide Linear Range：即使遇上表現量特別高或特別低的樣本時也不需要稀釋或濃縮
•Non-radioisotope：不用顧慮使用放射同位素的麻煩
•High Speed：可在一小時內快速完成試驗
試劑組內容物：
Galacto-Light or Galacto-Light Plus─
•Galacton or Galacton-Plus substrate
•Reaction Buffer Diluent
•Accelerator or Accelerator-II
•Lysis Buffer
Galacto-Star─
•Galacton-Star substrate
•Reaction Buffer Diluent containing Sapphire-II enhancer
•Mammalian or Yeast Lysis Buffer
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訂購資訊：
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目錄號碼
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產品名稱
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包裝
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T1006
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Galacto-Light |
600 microplate assays
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T1010
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Galacto-Light™ |
1,800 microplate assays
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T1008
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Galacto-Light™ |
15,000 microplate assays
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T1054
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Galacto-Light™
Reaction Buffer Diluent with Galacton®Substrate |
1,800 microplate assays
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T1007
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Galacto-Light
Plus™ |
600 microplate assays
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T1011
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Galacto-Light
Plus™ |
1,800 microplate assays
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T1009
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Galacto-Light
Plus™ |
15,000 microplate assays
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T1055
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Galacto-Light™
Reaction Buffer Diluent with Galacton-
Plus®Substrate |
1,800 microplate assays
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T2222
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Light
Emission Accelerator-II |
210 ml
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T1012
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Galacto-Star™
, Mammalian Cells |
600 microplate assays
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T1014
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Galacto-Star™
, Mammalian Cells |
1,800 microplate assays
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T1013
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Galacto-Star™
, Mammalian Cells |
15,000 microplate assays
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T1019
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Galacto-Star™
, Yeast Cells |
600 microplate assays
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T1021
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Galacto-Star™
, Yeast Cells |
1,800 microplate assays
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T1020
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Galacto-Star™
, Yeast Cells |
15,000 microplate assays
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T1056
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Galacto-Star™
Reaction Buffer Diluent with Galacton-
Star®Substrate |
1,800 microplate assays
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使用手冊
•User
Guide of Galacto-Light
•User
Guide of Galacto-Light Plus
•User
Guide of Galacto-Star
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