酵素活性或細胞功能分析實驗

 DELFIA 時差性螢光分析系統

 DELFIA(dissociation enhanced lanthanide FIA)

 
DELFIA(dissociation enhanced lanthanide FIA)是應用
 TRF(Time Resolved Fluorometry)偵測的技術。此系
 列的產品屬於異相(Heterogenous)分析的實驗系統,
 也就是需要清洗或分離的步驟。其實驗流程如左
 圖所示:













 DELFIA 的螯合物必須加入Enhancement Solution才
 能使Eu離子從原有的螯合物釋放至溶液中形成可
 產生螢光的結構,如下圖所示:





 DELFIA相關的應用領域

應用
優點
進一步資訊
General
•經過認證的技術
•高靈敏度
•高線性範圍
•可應用於多重標示
•Advice for setting up robust DELFIA
 binding assay (123-979)
•Multiplexing DELFIA assays using
 lanthanide-labeled probes (1234-9847)
•Applications of time-resolved fluormetry
 with the DELFIA method (1244-1126)
Immunoassays
•定量精準:高靈敏度、高線性範圍、
 數據連貫性高
•超過1,000篇的文獻
•How to optimize rapid and simple
 immunoassays (1234-976)
•DELFIA assays bring convenience in
 monoclonal antibody development
 (1234-966)
Binding assays
•高靈敏度
•適用多重標示

•Several published articles
Ligand-Receptor binding assays
•非放射性試劑
•高靈敏度即使細胞膜的受體較少
•搭配市售的受體已有完整的實驗流程
•The AcroWell plate: Low Fluorescence
 Background using the DELFIA system
 (1420-1000)
•DELFIA Ligand Guide (006783)
Kinase assays
•高靈敏度,節省酵素用量 •DELFIA protein kinase assays (1234-968)
•Sensitive DELFIA Abl tyrosine kniase
 assay using poly(Glu, Ala, Tyr) substrate
 (1234-9844)
Hybridization assays
•適合多重標定,節省工作量及時間;
 在長時間族群篩檢的實驗中適用此
 方法

•Eu-labelled oligonucleotides are stable
 and sensitive as probes and primers
 (1234-965)
Adherent cell assays
•在同一孔洞中偵測多達三種的表面抗
 原的表現量

• Several published articles
Cell adhesion assays
•非放射性試劑
•高靈敏度

•Cell adhesion assays (1234-969)
Cell cytotoxicity assays
•高靈敏度的方法可適用於necrosis, cell
 proliferation or apoptosis研究
•A new simplified, gentle cell-labelling
 method for nonradioactive cytotoxicity
 assays (1234-967)
•A new cell based DELFIA proliferation
 assay for measurement of DNA synthesis
 in microplate format (1234-9866)
•A new cell-based DNA fragmentation
 assay for testing apoptotic effect of lead
 compounds (1234-9864)
•A DELFIA assay for inhibition of PARP
 (1234-9865)
Functional cell assays
•非放射性且高靈敏度的GTP 結合測試 •A simple, more HTS-friendly DELFIA
 time-resolved fluorescence assay for
 camp determination (1234-9859)
•Time-resolved fluorescence-based GTP
 binding assay for G-Protein coupled
 receptors (1234-9858)

相關技術資訊:
下載時差式螢光(TRF)技術已發表的文獻
如需其他DELFIA應用資料,請洽伯森客服部
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